The Lepbase BLAST server uses the SequenceServer BLAST interface.
To begin, enter a query sequence in the large box at the top of the page. The sequence type will be detected automatically.
BLAST parameters can be adjusted by entering command line parameters in the box below the sequence entry.
Sequences for each assembly are separated into nucleotide (scaffold and CDS) and protein databases.
To find a specific database, either click through the hierarchy or start typing the name of an assembly you wish to select. Matching results will be automatically expanded and highlighted.
Select one or more blast databases to search.
The appropriate BLAST program will be selected automatically based on your query sequence and the type of database(s) selected. Click the button to run a BLAST search.
Once the BLAST search is complete, a summary of the top hits is displayed.
Click a link to go to the detailed view for that result.
The alignment and scores for each result are displayed in detail.
For each result, links are provided to view the target sequence, download in FASTA format or view the sequence in it's genomic context in the Lepbase Ensembl genome browser.